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CKAP2L

ANTIBODY INFORMATION
 
 

Antibody HPA039407

  

Antibody HPA040057

  

Antibody HPA070737

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039407   HPA040057   HPA070737  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 8   8   16  

References

      

Proper citation

 Atlas Antibodies Cat#HPA039407, RRID:AB_10794091   Atlas Antibodies Cat#HPA040057, RRID:AB_10794871   Atlas Antibodies Cat#HPA070737, RRID:AB_2686303  

VALIDATION SUMMARY
 
 

Antibody HPA039407

  

Antibody HPA040057

  

Antibody HPA070737

  
Immunocytochemistry

    Supported  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039407

  

Antibody HPA040057

  

Antibody HPA070737

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line A-431 shows localization to cytosol & mitotic spindle.  

Antibody dilution

     1:110  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039407

  

Antibody HPA040057

  

Antibody HPA070737

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in goblet cells.    

Expression

 RNA: detected in 16 tissues
Protein: detected in 72 cell types		   RNA: detected in 16 tissues
Protein: detected in 44 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:250   1:600    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA039407

  

Antibody HPA040057

  

Antibody HPA070737

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 83.6   83.6   83.6  

Antibody dilution

 1:250   1:250   1:370  

PROTEIN ARRAY
 
 

Antibody HPA039407

  

Antibody HPA040057

  

Antibody HPA070737

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:6000   1:4400  

ANTIGEN INFORMATION
 
 

Antibody HPA039407

  

Antibody HPA040057

  

Antibody HPA070737

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 96   115   106  

Antigen sequence

 
SSQVCIPQTSCVLQKSKAVSQRPNLTVGRFNSAIPSTPSIRPNGTSGNKH
NNNGFQQKTQTLDSKLKKAVPQNHFLNKTAPKTQADVTTVNGTQTN
  
MNNIHVENESLDNFLKETNKENLLDILTEPERKPDPKLYTRSKPKTDSYN
QTKNSLVPKQALGKSSVNSAVLKDRVNKQFVGETQSRTFPVKSQQLSRGA
DLARPGVKPSRTVPS
  
GITSDSLVAETSITSVEELAKKMESVKSCLSPKEREQVTATPRIAKAEQH
NYPGIKLQIGPIPRINGMPEVQDMKFITPVRRSSRIERAVSRYPEMLQEH
DLVVAS
 

Matching transcripts

 CKAP2L-001 - ENSP00000305204 [98%]
  CKAP2L-001 - ENSP00000305204 [100%]
  CKAP2L-001 - ENSP00000305204 [100%]
 

Other gene match

      
ANTIGEN VIEW
CKAP2L-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.