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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong positivity in a subset of cells in seminiferous ducts.
Expression
RNA: detected in 1 tissues Protein: detected in 16 cell types
RNA: detected in 1 tissues Protein: detected in 17 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:350
1:350
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006480
Antibody HPA025079
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409174)
Target mass (kDa)
41.7
41.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA006480
Antibody HPA025079
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
