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ANTIBODY INFORMATION
 
 

Antibody HPA002274

  

Antibody CAB025091

  

Antibody CAB072870

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002274   MAB16992   AMAb90911  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 2   6   14  

References

 2      

Proper citation

 Atlas Antibodies Cat#HPA002274, RRID:AB_1078435     Atlas Antibodies Cat#AMAb90911, RRID:AB_2665722  

VALIDATION SUMMARY
 
 

Antibody HPA002274

  

Antibody CAB025091

  

Antibody CAB072870

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002274

  

Antibody CAB025091

  

Antibody CAB072870

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in red pulp.   Immunohistochemical staining of human lymph node shows cytoplasmic positivity in leucocytes.   Immunohistochemical staining of human spleen shows cytoplasmic positivity in red and white pulp.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 10 cell types		   RNA: detected in 34 tissues
Protein: detected in 7 cell types		   RNA: detected in 34 tissues
Protein: detected in 19 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:2000   1:3500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002274

  

Antibody CAB025091

  

Antibody CAB072870

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 127.3, 127.2   127.3, 127.2, 18.1, 12.3   127.3, 127.2, 18.1, 12.3  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002274

  

Antibody CAB025091

  

Antibody CAB072870

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA002274

  

Antibody CAB025091

  

Antibody CAB072870

  

Antigen

 Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 135      

Antigen sequence

 
LNFTASENTSRVMQHQYQVSNLGQRSLPISLVFLVPVRLNQTVIWDRPQV
TFSENLSSTCHTKERLPSHSDFLAELRKAPVVNCSIAVCQRIQCDIPFFG
IQEEFNATLKGNLSFDWYIKTSHNHLLIVSTAEIL
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					ITGAM-001 - ENSP00000441691 [100%] 
ITGAM-002 - ENSP00000287497 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ITGAM-001
ITGAM-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002274

												
										 
																				
											
											
											
Antibody CAB025091

												
										 
																				
											
											
											
Antibody CAB072870

												
										 
																		

																	

										
											
1.

												
																						Halting angiogenesis by non-viral somatic gene therapy alleviates psoriasis and murine psoriasiform skin lesions

	Zibert JR et al

	J Clin Invest 2011;121(1):410-21

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						In situ deposition of complement in human acute brain ischaemia

	Pedersen ED et al

	Scand J Immunol 2009;69(6):555-62

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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