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ALCAM

ANTIBODY INFORMATION
 
 

Antibody HPA010926

  

Antibody CAB002148

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA010926   NCL-CD166  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 3   1  

References

 3    

Proper citation

 Atlas Antibodies Cat#HPA010926, RRID:AB_1078449    

VALIDATION SUMMARY
 
 

Antibody HPA010926

  

Antibody CAB002148

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010926

  

Antibody CAB002148

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human liver shows distinct membranous positivity in hepatocytes.   Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 47 cell types		   RNA: detected in 36 tissues
Protein: detected in 43 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:80  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010926

  

Antibody CAB002148

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 65.1, 63.7, 59.5   65.1, 63.7, 59.5, 38.1  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA010926

  

Antibody CAB002148

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA010926

  

Antibody CAB002148

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 141    

Antigen sequence

 
NITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCS
LIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATV
VWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKR
  

											 
																				

																		

										
											
Matching transcripts

												
																					ALCAM-001 - ENSP00000305988 [100%] 
ALCAM-002 - ENSP00000419236 [100%] 
ALCAM-005 - ENSP00000418213 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ALCAM-001
ALCAM-002
ALCAM-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA010926

												
										 
																				
											
											
											
Antibody CAB002148

												
										 
																		

																	

										
											
1.

												
																						Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse

	Kahlert C et al

	Br J Cancer 2009;101(3):457-64

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Membranous expression of activated leukocyte cell adhesion molecule contributes to poor prognosis and malignant phenotypes of non-small-cell lung cancer

	Ishiguro F et al

	J Surg Res 2013;179(1):24-32

			
											 
																						
											 
																				

																		

										
											
3.

												
																						Elevated ALCAM shedding in colorectal cancer correlates with poor patient outcome

	Hansen AG et al

	Cancer Res 2013;73(10):2955-64

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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