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EMX2

ANTIBODY INFORMATION
 
 

Antibody HPA003497

  

Antibody HPA065294

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003497   HPA065294  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   13  

References

    

Proper citation

   Atlas Antibodies Cat#HPA065294, RRID:AB_2685455  

VALIDATION SUMMARY
 
 

Antibody HPA003497

  

Antibody HPA065294

  
Immunocytochemistry

  Approved  
Immunohistochemistry

  Approved  
Western blot

  Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003497

  

Antibody HPA065294

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.  

Antibody dilution

   1:45  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003497

  

Antibody HPA065294

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human uterus shows moderate nuclear positivity in uterus.  

Expression

   RNA: detected in 17 tissues
Protein: detected in 19 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:45  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  
 

Standard validation

 Supported    

   

Figure description

 Distinct staining of neuronal processes and synapse in the hindbrain.    

WESTERN BLOT
 
 

Antibody HPA003497

  

Antibody HPA065294

  

Standard validation

   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

   28.3, 18.5  

Antibody dilution

   1:150  

PROTEIN ARRAY
 
 

Antibody HPA003497

  

Antibody HPA065294

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1800  

ANTIGEN INFORMATION
 
 

Antibody HPA003497

  

Antibody HPA065294

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 102   69  

Antigen sequence

 
ALSYANSSPINPFLNGFHSAAAAAAGRGVYSNPDLVFAEAVSHPPNPAVP
VHPVPPPHALAAHPLPSSHSPHPLFASQQRDPSTFYPWLIHRYRYLGHRF
QG
  
RGVYSNPDLVFAEAVSHPPNPAVPVHPVPPPHALAAHPLPSSHSPHPLFA
SQQRDPSTFYPWLIHRYRY
 

Matching transcripts

 EMX2-001 - ENSP00000450962 [100%]
EMX2-201 - ENSP00000474874 [100%]
  EMX2-001 - ENSP00000450962 [100%]
EMX2-201 - ENSP00000474874 [100%]
 

Other gene match

    
ANTIGEN VIEW
EMX2-001
EMX2-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.