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PLA2G1B

ANTIBODY INFORMATION
 
 

Antibody HPA047822

  

Antibody HPA060803

  

Antibody CAB022329

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA047822   HPA060803   MAB1668  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   12   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA047822, RRID:AB_2680169   Atlas Antibodies Cat#HPA060803, RRID:AB_2684369    

VALIDATION SUMMARY
 
 

Antibody HPA047822

  

Antibody HPA060803

  

Antibody CAB022329

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Supported   Supported    
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047822

  

Antibody HPA060803

  

Antibody CAB022329

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

   1:38   1:25  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047822

  

Antibody HPA060803

  

Antibody CAB022329

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.    

Expression

 RNA: detected in 18 tissues
Protein: detected in 31 cell types		   RNA: detected in 18 tissues
Protein: detected in 6 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:600   1:75    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA047822

  

Antibody HPA060803

  

Antibody CAB022329

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 16.4, 13.2   16.4, 7.6   16.4, 13.2, 7.6  

Antibody dilution

 1:110   1:130   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047822

  

Antibody HPA060803

  

Antibody CAB022329

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:1550    

ANTIGEN INFORMATION
 
 

Antibody HPA047822

  

Antibody HPA060803

  

Antibody CAB022329

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 78   39    

Antigen sequence

 
DNCYDQAKKLDSCKFLLDNPYTHTYSYSCSGSAITCSSKNKECEAFICNC
DRNAAICFSKAPYNKAHKNLDTKKYCQS
  
ADSGISPRAVWQFRKMIKCVIPGSDPFLEYNNYGCYCGL
  

											 
																				

																		

										
											
Matching transcripts

												
																					PLA2G1B-001 - ENSP00000312286 [100%] 
PLA2G1B-003 - ENSP00000447233 [100%] 

											 
																						PLA2G1B-001 - ENSP00000312286 [100%] 
PLA2G1B-002 - ENSP00000413594 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PLA2G1B-001
PLA2G1B-002
PLA2G1B-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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