FGA - Antibodies - The Human Protein Atlas

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FGA

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA051370

Antibody HPA064755

Antibody CAB016776

Provider

Atlas Antibodies
Sigma-Aldrich

BioPorto Diagnostics A/S

Product name

HPA051370

HPA064755

HYB 051-04

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA051370, RRID:AB_2681458

Atlas Antibodies Cat#HPA064755, RRID:AB_2685345

VALIDATION SUMMARY

Antibody HPA051370

Antibody HPA064755

Antibody CAB016776

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA051370

Antibody HPA064755

Antibody CAB016776

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA064755 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA051370 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human small intestine shows distinct positivity in plasma.

Immunohistochemical staining of human nasopharynx shows strong cytoplasmic and membranous positivity in respiratory epithelial cells.

Immunohistochemical staining of human duodenum shows distinct positivity in plasma.

Expression

RNA: detected in 10 tissues
Protein: detected in 7 cell types

RNA: detected in 10 tissues
Protein: detected in 12 cell types

RNA: detected in 10 tissues
Protein: detected in 10 cell types

Retrieval

HIER pH6

Antibody dilution

1:2500

1:1400

1:1500

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

No internal RNA expression data available for correlation.

WESTERN BLOT

Antibody HPA051370

Antibody HPA064755

Antibody CAB016776

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

95, 69.8

95, 69.8, 33

Antibody dilution

1:280

1:500

PROTEIN ARRAY

Antibody HPA051370

Antibody HPA064755

Antibody CAB016776

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2250

1:6050

ANTIGEN INFORMATION

Antibody HPA051370

Antibody HPA064755

Antibody CAB016776

Antigen

Recombinant protein fragment

Native protein

Length (aa)

Antigen sequence

GHWTSESSVSGSTGQWHSESGSFRPDSPGSGNARPNNPDWGTFEEVSGNV
SPGTRREYHTEKLVTSKGDKELRTGKEKVTSGSTTTTRRSCSKTVTKT

SHHPGIAEFPSRGKSSSYSKQFTSSTSYNRGDSTFESKSYKMADEAGSEA
DHEGTHSTKRGHAKSRPVRDCDDVLQTHPSGTQSGIFNIKLP

Matching transcripts

FGA-001 - ENSP00000385981 [100%]
FGA-002 - ENSP00000306361 [100%]

FGA-002 - ENSP00000306361 [100%]

Other gene match

ANTIGEN VIEW

FGA-001	FGA-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.