We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.
Antibody dilution
1:200
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA028470
Antibody HPA028484
Antibody CAB037141
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA028484 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA028470 across 45 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human esophagus shows strong nucleolar positivity in superficial squamous epithelial cells.
Immunohistochemical staining of human pancreas shows moderate nucleolar positivity which was combined with distinct cytoplasmic staining in intercalated ducts.
Immunohistochemical staining of human esophagus shows strong nucleolar and cytoplasmic positivity in squamous epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 57 cell types
RNA: detected in 37 tissues Protein: detected in 65 cell types
RNA: detected in 37 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:25
1:175
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028470
Antibody HPA028484
Antibody CAB037141
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424312)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424312)
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
100.8, 98.1
100.8, 98.1
100.8, 98.1
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA028470
Antibody HPA028484
Antibody CAB037141
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
