Fields »
ABOUT
HELP
BLOG
BSG

ANTIBODY INFORMATION
 
 

Antibody HPA036048

  

Antibody HPA074870

  

Antibody CAB002427

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA036048   HPA074870   NCL-CD147  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 12   16   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA036048, RRID:AB_2674917   Atlas Antibodies Cat#HPA074870, RRID:AB_2686719    

VALIDATION SUMMARY
 
 

Antibody HPA036048

  

Antibody HPA074870

  

Antibody CAB002427

  
Immunocytochemistry

  Supported    
Immunohistochemistry

Supported     Supported  
Western blot

Supported   Supported   Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036048

  

Antibody HPA074870

  

Antibody CAB002427

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.    

Antibody dilution

   1:38    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036048

  

Antibody HPA074870

  

Antibody CAB002427

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong positivity in cells in seminiferous ducts.     Immunohistochemical staining of human testis shows strong membranous positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 31 cell types		     RNA: detected in 37 tissues
Protein: detected in 39 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:4500     1:40  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036048

  

Antibody HPA074870

  

Antibody CAB002427

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 42.2, 29.2, 23.9, 17.4   42.2   42.2, 29.2, 23.9, 22.8, 20.5, 19.4, 17.4, 9.3, 5.7, 5.5  

Antibody dilution

 1:250   1:130   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036048

  

Antibody HPA074870

  

Antibody CAB002427

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1550    

ANTIGEN INFORMATION
 
 

Antibody HPA036048

  

Antibody HPA074870

  

Antibody CAB002427

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 142   53    

Antigen sequence

 
DLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQ
WGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESV
PPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIEN
  
EIQWWFEGQGPNDTCSQLWDGARLDRVHIHATYHQHAASTISIDTLVEED
TGT
  

											 
																				

																		

										
											
Matching transcripts

												
																					BSG-001 - ENSP00000333769 [100%] 
BSG-003 - ENSP00000343809 [100%] 
BSG-014 - ENSP00000484849 [100%] 
BSG-007 - ENSP00000458665 [97%] 

											 
																						BSG-001 - ENSP00000333769 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
BSG-001
BSG-003
BSG-007
BSG-014
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.