SUCLG2 - Antibodies - The Human Protein Atlas

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SUCLG2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA046705

Antibody HPA051998

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA046705

HPA051998

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA046705, RRID:AB_2679762

Atlas Antibodies Cat#HPA051998, RRID:AB_2681687

VALIDATION SUMMARY

Antibody HPA046705

Antibody HPA051998

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Supported

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA046705

Antibody HPA051998

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.

Antibody dilution

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA046705

Antibody HPA051998

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA051998 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA046705 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA051998 across 70 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA046705 across 70 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 54 cell types

RNA: detected in 37 tissues
Protein: detected in 57 cell types

Retrieval

HIER pH6

Antibody dilution

1:10000

1:500

Literature conformity

Partly consistent with gene/protein characterization data.

Consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA046705

Antibody HPA051998

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

46.5, 20.1

47.7, 46.5, 41.4

Antibody dilution

1:1660

1:1080

PROTEIN ARRAY

Antibody HPA046705

Antibody HPA051998

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:13300

1:8600

ANTIGEN INFORMATION

Antibody HPA046705

Antibody HPA051998

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

FGGIVNCAIIANGITKACRELELKVPLVVRLEGTNVQEAQKILNNSGLPI
TSAIDLEDAAKKAVASVAKK

AADQITKLYNLFLKIDATQVEVNPFGETPEGQVVCFDAKINFDDNAEFRQ
KDIFAMDDKSENEPIENEAAKYDL

Matching transcripts

SUCLG2-001 - ENSP00000307432 [100%]
SUCLG2-004 - ENSP00000417260 [100%]

SUCLG2-001 - ENSP00000307432 [100%]
SUCLG2-002 - ENSP00000419325 [100%]
SUCLG2-003 - ENSP00000417589 [100%]

Other gene match

ANTIGEN VIEW

SUCLG2-001	SUCLG2-002	SUCLG2-003	SUCLG2-004

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.