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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Antibody dilution
1:65
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA053880
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human spleen shows strong nuclear and cytoplasmic staining in red pulp of spleen.
Expression
RNA: detected in 35 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA053880
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24.9, 22
Antibody dilution
1:330
PROTEIN ARRAY
Antibody HPA053880
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.