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Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in a subset of glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in a few glandular cells.
Expression
RNA: detected in 2 tissues Protein: detected in 3 cell types
RNA: detected in 2 tissues Protein: detected in 5 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:200
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030100
Antibody CAB033849
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417280)
Target mass (kDa)
15.3
15.3
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA030100
Antibody CAB033849
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
