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CORO1B

ANTIBODY INFORMATION
 
 

Antibody HPA040113

  

Antibody HPA070456

  

Antibody CAB017616

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA040113   HPA070456   sc-66838  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   14   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA040113, RRID:AB_2676850   Atlas Antibodies Cat#HPA070456, RRID:AB_2686268   Santa Cruz Biotechnology Cat#sc-66838, RRID:AB_2087018  

VALIDATION SUMMARY
 
 

Antibody HPA040113

  

Antibody HPA070456

  

Antibody CAB017616

  
Immunocytochemistry

Approved     Approved  
Immunohistochemistry

  Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040113

  

Antibody HPA070456

  

Antibody CAB017616

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.     Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.  

Antibody dilution

 1:200     1:75  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040113

  

Antibody HPA070456

  

Antibody CAB017616

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunohistochemical staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human prostate shows strong positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 53 cell types		   RNA: detected in 37 tissues
Protein: detected in 59 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:1500   1:300  

Literature conformity

   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040113

  

Antibody HPA070456

  

Antibody CAB017616

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 54.2   54.2   54.2, 31, 15.3  

Antibody dilution

 1:250   1:220   1:500  

PROTEIN ARRAY
 
 

Antibody HPA040113

  

Antibody HPA070456

  

Antibody CAB017616

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000   1:2700    

ANTIGEN INFORMATION
 
 

Antibody HPA040113

  

Antibody HPA070456

  

Antibody CAB017616

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 94   94    

Antigen sequence

 
AYVPSKQRDLKISRRNVLSDSRPAMAPGSSHLGAPASTTTAAGATPSGSL
ARAGEAGKLEEVMQELRALRALVKEQGDRICRLEEQLGRMENGD
  
AYVPSKQRDLKISRRNVLSDSRPAMAPGSSHLGAPASTTTAAGATPSGSL
ARAGEAGKLEEVMQELRALRALVKEQGDRICRLEEQLGRMENGD
   

Matching transcripts

 CORO1B-001 - ENSP00000340211 [99%]
CORO1B-201 - ENSP00000377471 [99%]
  CORO1B-001 - ENSP00000340211 [99%]
CORO1B-201 - ENSP00000377471 [99%]
   

Other gene match

      
ANTIGEN VIEW
CORO1B-001
CORO1B-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.