We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & cytosol.
Antibody dilution
1:25
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA043820
Antibody HPA046792
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human lateral ventricle shows moderate positivity in glial cells.
Immunohistochemical staining of human lateral ventricle shows strong positivity in astrocytes.
Expression
RNA: detected in 25 tissues Protein: detected in 69 cell types
RNA: detected in 25 tissues Protein: detected in 30 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:100
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043820
Antibody HPA046792
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413128)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
79.4
79.4, 30.9
Antibody dilution
1:125
1:130
PROTEIN ARRAY
Antibody HPA043820
Antibody HPA046792
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
