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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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CSPG4
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

CSPG4

Gene description

Chondroitin sulfate proteoglycan 4

Protein class

Predicted membrane proteins

Predicted localization

Membrane

Number of transcripts

1
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (SK-MEL-30, U-138 MG, WM-115)
HPA (normal tissue):Mixed

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Ubiquitous granular cytoplasmic expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data.

Reliability score

Approved based on 3 antibodies.
HPA002951 , HPA042785 , CAB016189
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable) and urothelial cancer (unfavourable).
Renal cancer p<0.001
Urothelial cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA002951
HPA042785
CAB016189

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer tissues displayed moderate to strong cytoplasmic positivity with additioanl membranous staining in a few cases.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer tissues displayed moderate to strong cytoplasmic positivity. Renal cancers were generally negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer tissues displayed moderate cytoplasmic positivity. Colorectal, ovarian, thyroid, pancreatic and cholangiocarcinomas showed strong staining.

GENE INFORMATION

Gene name

CSPG4 (HGNC Symbol)

Synonyms

HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2

Description

Chondroitin sulfate proteoglycan 4 (HGNC Symbol)

Entrez gene summary

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Chromosome

15

Cytoband

q24.2

Chromosome location (bp)

75674322 - 75712848

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000173546 (version 83.38)

Entrez gene

1464

UniProt

Q6UVK1 (UniProt - Evidence at protein level)

neXtProt

NX_Q6UVK1

Antibodypedia

CSPG4 antibodies

PROTEIN BROWSER

CSPG4-001

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

CSPG4-001
ENSP00000312506
ENST00000308508
Q6UVK1 [Direct mapping]
Chondroitin sulfate proteoglycan 4
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Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000187 [activation of MAPK activity]
GO:0001525 [angiogenesis]
GO:0004871 [signal transducer activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005796 [Golgi lumen]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0005925 [focal adhesion]
GO:0005975 [carbohydrate metabolic process]
GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway]
GO:0008283 [cell proliferation]
GO:0008347 [glial cell migration]
GO:0009986 [cell surface]
GO:0016324 [apical plasma membrane]
GO:0016477 [cell migration]
GO:0019901 [protein kinase binding]
GO:0030203 [glycosaminoglycan metabolic process]
GO:0030204 [chondroitin sulfate metabolic process]
GO:0030206 [chondroitin sulfate biosynthetic process]
GO:0030207 [chondroitin sulfate catabolic process]
GO:0030208 [dermatan sulfate biosynthetic process]
GO:0031258 [lamellipodium membrane]
GO:0035556 [intracellular signal transduction]
GO:0042995 [cell projection]
GO:0043202 [lysosomal lumen]
GO:0044281 [small molecule metabolic process]
GO:0048771 [tissue remodeling]
GO:0050731 [positive regulation of peptidyl-tyrosine phosphorylation]
GO:0070062 [extracellular exosome]
Show all
2322 aa
250.5 kDa
Yes 1

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