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ANTIBODY INFORMATION
 
 

Antibody HPA043922

  

Antibody HPA058765

  

Antibody CAB033742

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA043922   HPA058765   sc-67021  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   13   7  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA043922, RRID:AB_2678732   Atlas Antibodies Cat#HPA058765, RRID:AB_2683812   Santa Cruz Biotechnology Cat#sc-67021, RRID:AB_2283532  

VALIDATION SUMMARY
 
 

Antibody HPA043922

  

Antibody HPA058765

  

Antibody CAB033742

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry

Uncertain     Uncertain  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043922

  

Antibody HPA058765

  

Antibody CAB033742

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA058765.		  
Validated

Antibody staining overlaps with antibody HPA043922.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to mitochondria.   Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:45   1:53   1:13  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043922

  

Antibody HPA058765

  

Antibody CAB033742

  

Standard validation

 Uncertain     Uncertain  

     

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.     Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 55 cell types		     RNA: detected in 37 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:75     1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043922

  

Antibody HPA058765

  

Antibody CAB033742

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 129.6   129.6, 32.7   129.6, 53.4, 32.7  

Antibody dilution

 1:250   1:180   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043922

  

Antibody HPA058765

  

Antibody CAB033742

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2150    

ANTIGEN INFORMATION
 
 

Antibody HPA043922

  

Antibody HPA058765

  

Antibody CAB033742

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 88   97    

Antigen sequence

 
GADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWE
GARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQ
  
FAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVS
DLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIG
  

											 
																				

																		

										
											
Matching transcripts

												
																					PC-001 - ENSP00000377530 [100%] 
PC-005 - ENSP00000377527 [100%] 
PC-201 - ENSP00000377532 [100%] 

											 
																						PC-001 - ENSP00000377530 [100%] 
PC-005 - ENSP00000377527 [100%] 
PC-011 - ENSP00000435905 [100%] 
PC-201 - ENSP00000377532 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PC-001
PC-005
PC-011
PC-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA043922

												
										 
																				
											
											
											
Antibody HPA058765

												
										 
																				
											
											
											
Antibody CAB033742

												
										 
																		

																	

										
											
1.

												
																						Pyruvate carboxylation enables growth of SDH-deficient cells by supporting aspartate biosynthesis

	Cardaci S et al

	Nat Cell Biol 2015;17(10):1317-26

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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