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PSMD1

ANTIBODY INFORMATION
 
 

Antibody HPA036736

  

Antibody HPA036737

  

Antibody CAB021092

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   GenWay Biotech
  

Product name

 HPA036736   HPA036737   15-288-22830  

Host species

 Rabbit   Rabbit   Hen  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 12   13   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA036736, RRID:AB_2675278   Atlas Antibodies Cat#HPA036737, RRID:AB_2675279   GenWay Biotech Inc. Cat#15-288-22830, RRID:AB_10123242  

VALIDATION SUMMARY
 
 

Antibody HPA036736

  

Antibody HPA036737

  

Antibody CAB021092

  
Immunocytochemistry

Approved      
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036736

  

Antibody HPA036737

  

Antibody CAB021092

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.      

Antibody dilution

 1:70      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036736

  

Antibody HPA036737

  

Antibody CAB021092

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.   Immunohistochemical staining of human hippocampus shows strong nuclear and cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 80 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:10   1:750  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036736

  

Antibody HPA036737

  

Antibody CAB021092

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 105.8, 102.3, 23.1   105.8, 102.3   105.8, 102.3, 23.1, 20.3, 18.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036736

  

Antibody HPA036737

  

Antibody CAB021092

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA036736

  

Antibody HPA036737

  

Antibody CAB021092

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 85   76    

Antigen sequence

 
GAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEG
EKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALE
  
CAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQF
RQLYSKVINDKHDDVMAKFGAILAQG
   

Matching transcripts

 PSMD1-001 - ENSP00000309474 [100%]
PSMD1-003 - ENSP00000386932 [100%]
PSMD1-201 - ENSP00000362738 [100%]
PSMD1-202 - ENSP00000479986 [100%]
PSMD1-005 - ENSP00000406141 [93%]
  PSMD1-001 - ENSP00000309474 [100%]
PSMD1-003 - ENSP00000386932 [100%]
PSMD1-201 - ENSP00000362738 [100%]
PSMD1-202 - ENSP00000479986 [100%]
   

Other gene match

      
ANTIGEN VIEW
PSMD1-001
PSMD1-003
PSMD1-005
PSMD1-201
PSMD1-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.