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ADGRG2

ANTIBODY INFORMATION
 
 

Antibody HPA001478

  

Antibody HPA050029

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001478   HPA050029  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 1   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA001478, RRID:AB_1078923   Atlas Antibodies Cat#HPA050029, RRID:AB_2680996  

VALIDATION SUMMARY
 
 

Antibody HPA001478

  

Antibody HPA050029

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001478

  

Antibody HPA050029

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.    

Antibody dilution

 1:50    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001478

  

Antibody HPA050029

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human epididymis shows strong membranous and moderate nuclear positivity in glandular cells.   Immunohistochemical staining of human epididymis shows moderate membranous and nuclear positivity in glandular cells.  

Expression

 RNA: detected in 19 tissues
Protein: detected in 5 cell types		   RNA: detected in 19 tissues
Protein: detected in 4 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1600   1:3400  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001478

  

Antibody HPA050029

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 111.6, 111.3, 110, 109.2, 108.5, 106.4, 98.7   111.6, 111.3, 110, 109.2, 108.5, 106.4, 98.7  

Antibody dilution

 1:500   1:100  

PROTEIN ARRAY
 
 

Antibody HPA001478

  

Antibody HPA050029

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2300  

ANTIGEN INFORMATION
 
 

Antibody HPA001478

  

Antibody HPA050029

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 137   128  

Antigen sequence

 
SPTVSAPANVNTTSAPPVQTDIVNTSSISDLENQVLQMEKALSLGSLEPN
LAGEMINQVSRLLHSPPDMLAPLAQRLLKVVDDIGLQLNFSNTTISLTSP
SLALAVIRVNASSFNTTTFVAQDPANLQVSLETQAPE
  
NASGVKPQRNICNLSSICNDSAFFRGEIMFQYDKESTVPQNQHITNGTLT
GVLSLSELKRSELNKTLQTLSETYFIMCATAEAQSTLNCTFTIKLNNTMN
ACAVIAALERVKIRPMEHCCCSVRIPCP
 

Matching transcripts

 ADGRG2-001 - ENSP00000369202 [100%]
ADGRG2-002 - ENSP00000350152 [100%]
ADGRG2-003 - ENSP00000369198 [100%]
ADGRG2-005 - ENSP00000353421 [100%]
ADGRG2-006 - ENSP00000350680 [100%]
ADGRG2-007 - ENSP00000349015 [100%]
ADGRG2-202 - ENSP00000369205 [100%]
ADGRG2-203 - ENSP00000369207 [100%]
ADGRG2-008 - ENSP00000344972 [94%]
ADGRG2-201 - ENSP00000346845 [94%]
  ADGRG2-001 - ENSP00000369202 [100%]
ADGRG2-003 - ENSP00000369198 [100%]
ADGRG2-006 - ENSP00000350680 [100%]
ADGRG2-202 - ENSP00000369205 [100%]
ADGRG2-203 - ENSP00000369207 [100%]
ADGRG2-002 - ENSP00000350152 [98%]
ADGRG2-005 - ENSP00000353421 [98%]
ADGRG2-007 - ENSP00000349015 [98%]
ADGRG2-008 - ENSP00000344972 [98%]
ADGRG2-201 - ENSP00000346845 [98%]
 

Other gene match

    
ANTIGEN VIEW
ADGRG2-001
ADGRG2-002
ADGRG2-003
ADGRG2-005
ADGRG2-006
ADGRG2-007
ADGRG2-008
ADGRG2-201
ADGRG2-202
ADGRG2-203
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.