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STAT5B

ANTIBODY INFORMATION
 
 

Antibody HPA042128

  

Antibody HPA049883

  

Antibody HPA051156

  

Antibody CAB004298

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA042128   HPA049883   HPA051156   sc-1656  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 STAT5A - ENSG00000126561 [100%]
   STAT5A - ENSG00000126561 [100%]
   STAT5A - ENSG00000126561 [100%]
    

Released in version

 13   13   13   3  

References

        

Proper citation

 Atlas Antibodies Cat#HPA042128, RRID:AB_2677864   Atlas Antibodies Cat#HPA049883, RRID:AB_2680931   Atlas Antibodies Cat#HPA051156, RRID:AB_2681366   Santa Cruz Biotechnology Cat#sc-1656, RRID:AB_2197067  

VALIDATION SUMMARY
 
 

Antibody HPA042128

  

Antibody HPA049883

  

Antibody HPA051156

  

Antibody CAB004298

  
Immunohistochemistry

Approved   Approved   Approved   Approved  
Western blot

Uncertain   Supported   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042128

  

Antibody HPA049883

  

Antibody HPA051156

  

Antibody CAB004298

  

Standard validation

 Approved   Approved   Approved   Approved  

       

Figure description

 Immunohistochemical staining of human lymph node shows strong nuclear positivity in non-germinal center cells.   Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in subsets of lymphoid cells.   Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in subsets of lymphoid cells.   Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 20 cell types		   RNA: detected in 37 tissues
Protein: detected in 47 cell types		   RNA: detected in 37 tissues
Protein: detected in 47 cell types		   RNA: detected in 37 tissues
Protein: detected in 53 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:85   1:200   1:2000  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042128

  

Antibody HPA049883

  

Antibody HPA051156

  

Antibody CAB004298

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 90.6, 89.9, 87.4, 86.9   90.6, 89.9, 87.4, 86.9   90.6, 89.9, 87.4, 86.9, 16.7   89.9, 18  

Antibody dilution

 1:250   1:260   1:190   1:500  

PROTEIN ARRAY
 
 

Antibody HPA042128

  

Antibody HPA049883

  

Antibody HPA051156

  

Antibody CAB004298

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2100   1:1550    

ANTIGEN INFORMATION
 
 

Antibody HPA042128

  

Antibody HPA049883

  

Antibody HPA051156

  

Antibody CAB004298

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 74   48   49    

Antigen sequence

 
EGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNA
TITDIISALVTSTFIIEKQPPQVL
  
KTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRN
  
LEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					STAT5B-001 - ENSP00000293328 [100%] 

											 
																						STAT5B-001 - ENSP00000293328 [100%] 

											 
																						STAT5B-001 - ENSP00000293328 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					STAT5A - ENSG00000126561 [100%]

											 
																						STAT5A - ENSG00000126561 [100%]

											 
																						STAT5A - ENSG00000126561 [100%]

											 
																						
											 
																				

																		

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