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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of cells in germinal and non-germinal center cells.
Immunohistochemical staining of human duodenum shows distinct positivity in inflammatory cells.
Expression
RNA: detected in 25 tissues Protein: detected in 7 cell types
RNA: detected in 25 tissues Protein: detected in 1 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:20
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039079
Antibody CAB002594
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
25.4, 23, 14.8, 12.4
25.4, 23, 17.2, 14.8, 12.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA039079
Antibody CAB002594
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
