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Immunohistochemical staining of human heart muscle shows distinct cytoplasmic positivity.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 24 tissues Protein: detected in 31 cell types
RNA: detected in 24 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:45
1:15
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA026477
Antibody CAB004591
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401215)
Target mass (kDa)
19.1
19.1, 16.2
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA026477
Antibody CAB004591
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
