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ATP2A2

ANTIBODY INFORMATION
 
 

Antibody HPA062605

  

Antibody HPA067892

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA062605   HPA067892  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA062605, RRID:AB_2684805   Atlas Antibodies Cat#HPA067892, RRID:AB_2685919  

VALIDATION SUMMARY
 
 

Antibody HPA062605

  

Antibody HPA067892

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported    
Western blot

Supported   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA062605

  

Antibody HPA067892

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA067892.		  
Validated

Antibody staining overlaps with antibody HPA062605.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.  

Antibody dilution

 1:56   1:42  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA062605

  

Antibody HPA067892

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 31 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:550    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA062605

  

Antibody HPA067892

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 114.8, 109.7   114.8, 109.7, 102.5  

Antibody dilution

 1:190   1:140  

PROTEIN ARRAY
 
 

Antibody HPA062605

  

Antibody HPA067892

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2200   1:1700  

ANTIGEN INFORMATION
 
 

Antibody HPA062605

  

Antibody HPA067892

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 36   32  

Antigen sequence

 
TVEEVLGHFGVNESTGLSLEQVKKLKERWGSNELPA
  
AWWFIAADGGPRVSFYQLSHFLQCKEDNPDFE
 

Matching transcripts

 ATP2A2-001 - ENSP00000311186 [100%]
ATP2A2-003 - ENSP00000440045 [100%]
  ATP2A2-001 - ENSP00000311186 [100%]
ATP2A2-003 - ENSP00000440045 [100%]
ATP2A2-006 - ENSP00000449454 [100%]
 

Other gene match

    
ANTIGEN VIEW
ATP2A2-001
ATP2A2-003
ATP2A2-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.