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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and distinct staining of neuropil.
Expression
RNA: detected in 6 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002739
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402278)
Target mass (kDa)
148.2, 43
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA002739
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
