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Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nuclear bodies.
Antibody dilution
1:38
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA043524
Standard validation
Supported
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells.
Expression
RNA: detected in 33 tissues Protein: detected in 58 cell types
Retrieval
HIER pH6
Antibody dilution
1:60
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043524
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405827)
Target mass (kDa)
36.4
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA043524
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
