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Antibody staining overlaps with GFP tagged protein
Validated
Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in plasma membrane, cytoplasm & nucleus but excluded from the nucleoli and GFP expression in plasma membrane & cytoplasm.
Immunofluorescent staining of transgenic HeLa cells show antibody staining in plasma membrane, mitochondria & nucleus but excluded from the nucleoli and GFP expression in plasma membrane & cytoplasm.
Antibody dilution
1:200
1:200
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & mitochondria.
Antibody dilution
1:200
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA040416
Antibody HPA040434
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 76 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:350
1:1700
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040416
Antibody HPA040434
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
68.9, 60, 36.6, 20.7, 19.2, 18.6, 16.3, 11.5
68.9, 60, 57.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA040416
Antibody HPA040434
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.