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Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:61
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA006083
Antibody HPA007830
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells and cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 74 cell types
RNA: detected in 37 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:5
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006083
Antibody HPA007830
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412613)
Target mass (kDa)
41.3, 41.2, 36.8, 22.8
41.3, 41.2, 36.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA006083
Antibody HPA007830
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.