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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.
Antibody dilution
1:72
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA030685
Antibody HPA046495
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity with a granular pattern in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 67 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030685
Antibody HPA046495
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
96.8, 95, 93, 55.4, 51.5
96.8, 14.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA030685
Antibody HPA046495
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
