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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Antibody dilution
1:65
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA052590
Antibody HPA053608
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 28 cell types
RNA: detected in 37 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:400
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052590
Antibody HPA053608
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
33.2
33.2, 16.4, 14
Antibody dilution
1:330
1:260
PROTEIN ARRAY
Antibody HPA052590
Antibody HPA053608
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.