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Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil, neuronal and glial cells.
Expression
RNA: detected in 35 tissues Protein: detected in 42 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045218
Antibody HPA050333
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416732)
Target mass (kDa)
22.7, 4.9
22.7
Antibody dilution
1:250
1:200
PROTEIN ARRAY
Antibody HPA045218
Antibody HPA050333
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
