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Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:6
Literature conformity
The subcellular location is not consistent with literature.
WESTERN BLOT
Antibody HPA042249
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
73.1, 72, 68.3, 55.2, 13.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA042249
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
