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ANTIBODY INFORMATION
 
 

Antibody HPA021282

  

Antibody HPA059381

  

Antibody CAB025541

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA021282   HPA059381   MAB34381  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 5   13   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA021282, RRID:AB_1847673   Atlas Antibodies Cat#HPA059381, RRID:AB_2683999    

VALIDATION SUMMARY
 
 

Antibody HPA021282

  

Antibody HPA059381

  

Antibody CAB025541

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

Supported     Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA021282

  

Antibody HPA059381

  

Antibody CAB025541

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.   Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.    

Antibody dilution

 1:32   1:22    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021282

  

Antibody HPA059381

  

Antibody CAB025541

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.     Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 63 cell types		     RNA: detected in 37 tissues
Protein: detected in 34 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:150     1:1500  

Literature conformity

 Consistent with gene/protein characterization data.     Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA021282

  

Antibody HPA059381

  

Antibody CAB025541

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 54.3   54.3, 26.6   54.3, 29.4, 26.6  

Antibody dilution

 1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA021282

  

Antibody HPA059381

  

Antibody CAB025541

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:600    

ANTIGEN INFORMATION
 
 

Antibody HPA021282

  

Antibody HPA059381

  

Antibody CAB025541

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 71   74    

Antigen sequence

 
MFARNAFTVLAMMDYPYPTDFLGPLPANPVKVGCDRLLSEAQRITGLRAL
AGLVYNASGSEHCYDIYRLYH
  
SNGNLDPWAGGGIRRNLSASVIAVTIQGGAHHLDLRASHPEDPASVVEAR
KLEATIIGEWVKAARREQQPALRG
  

											 
																				

																		

										
											
Matching transcripts

												
																					DPP7-003 - ENSP00000360635 [100%] 

											 
																						DPP7-003 - ENSP00000360635 [100%] 
DPP7-008 - ENSP00000473309 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DPP7-003
DPP7-008
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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