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Immunohistochemical staining of human testis shows distinct strong positivity in seminiferous ducts.
Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in fractions of glandular cells.
Expression
RNA: detected in 1 tissues Protein: detected in 60 cell types
RNA: detected in 1 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:6000
Literature conformity
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028956
Antibody CAB037244
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
19.7, 19.6
19.6
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA028956
Antibody CAB037244
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
