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NR2C2

ANTIBODY INFORMATION
 
 

Antibody HPA006313

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA006313  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

  

Proper citation

 Atlas Antibodies Cat#HPA006313, RRID:AB_1079503  

VALIDATION SUMMARY
 
 

Antibody HPA006313

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006313

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA and > 10% by the other.		  

 

Figure description

 Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:43  
 

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:45  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006313

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human endometrium shows nuclear positivity in glandular cells and cels in the endometrial stroma.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:50  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006313

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 67.3, 65.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA006313

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA006313

  

Antigen

 Recombinant protein fragment  

Length (aa)

 147  

Antigen sequence

 
DGARQTGLLDPGMLVNIQQPLIREDGTVLLATDSKAETSQGALGTLANVV
TSLANLSESLNNGDTSEIQPEDQSASEITRAFDTLAKALNTTDSSSSPSL
ADGIDTSGGGSIHVISRDQSTPIIEVEGPLLSDTHVTFKLTMPSPMP
 

Matching transcripts

 NR2C2-001 - ENSP00000320447 [100%]
NR2C2-002 - ENSP00000388387 [100%]
NR2C2-004 - ENSP00000376814 [100%]
NR2C2-201 - ENSP00000384463 [100%]
NR2C2-202 - ENSP00000483059 [100%]
 

Other gene match

  
ANTIGEN VIEW
NR2C2-001
NR2C2-002
NR2C2-004
NR2C2-201
NR2C2-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.