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Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
Antibody dilution
1:179
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA046404
Antibody HPA051974
Antibody CAB002432
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in Kupffer cells.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Expression
RNA: detected in 36 tissues Protein: detected in 21 cell types
RNA: detected in 36 tissues Protein: detected in 11 cell types
RNA: detected in 36 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1600
1:300
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046404
Antibody HPA051974
Antibody CAB002432
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
125.4
125.4, 124.9, 121.6, 120.3
125.4, 124.9, 121.6, 120.3, 15, 13.4
Antibody dilution
1:250
1:130
1:500
PROTEIN ARRAY
Antibody HPA046404
Antibody HPA051974
Antibody CAB002432
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
