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NAALADL2

ANTIBODY INFORMATION
 
 

Antibody HPA012413

  

Antibody HPA017425

  

Antibody CAB025586

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA012413   HPA017425   AF4665  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 5   5   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA012413, RRID:AB_1854274   Atlas Antibodies Cat#HPA017425, RRID:AB_1854275    

VALIDATION SUMMARY
 
 

Antibody HPA012413

  

Antibody HPA017425

  

Antibody CAB025586

  
Immunohistochemistry


Validated
  

Validated
   Approved  
Western blot

Uncertain   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012413

  

Antibody HPA017425

  

Antibody CAB025586

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA017425 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA012413 across 72 cell types.		    
 

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong membranous positivity in syncytiotrophoblasts.   Immunohistochemical staining of human placenta shows membranous positivity in trophoblastic cells.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 67 cell types		   RNA: detected in 32 tissues
Protein: detected in 27 cell types		   RNA: detected in 32 tissues
Protein: detected in 27 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:10   1:40  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012413

  

Antibody HPA017425

  

Antibody CAB025586

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 88.7, 87.5   88.7, 87.5   88.7, 87.5, 13.1  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA012413

  

Antibody HPA017425

  

Antibody CAB025586

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA012413

  

Antibody HPA017425

  

Antibody CAB025586

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 132   111    

Antigen sequence

 
PVASPSLQQLVVEKNNFNCTRRAQCPETNISSIQIQGDADYFINHLGVPI
VQFAYEDIKTLEGPSFLSEARFSTRATKIEEMDPSFNLHETITKLSGEVI
LQIANEPVLPFNALDIALEVQNNLKGDQPNTH
  
TQWTSLGLEDVQFVNYSVLLDLPGPSPSTVTLSSSGQCFHPNGQPCSEEA
RKDSSQDLLYSYAAYSAKGTLKAEVIDVSYGMADDLKRIRKIKNVTNQIA
LLKLGKLPLLY
  

											 
																				

																		

										
											
Matching transcripts

												
																					NAALADL2-001 - ENSP00000404705 [100%] 
NAALADL2-201 - ENSP00000479989 [100%] 

											 
																						NAALADL2-001 - ENSP00000404705 [100%] 
NAALADL2-201 - ENSP00000479989 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NAALADL2-001
NAALADL2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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