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ANTIBODY INFORMATION
 
 

Antibody HPA020078

  

Antibody HPA021141

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020078   HPA021141  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA020078, RRID:AB_2670352   Atlas Antibodies Cat#HPA021141, RRID:AB_2670599  

VALIDATION SUMMARY
 
 

Antibody HPA020078

  

Antibody HPA021141

  
Immunocytochemistry

Approved    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020078

  

Antibody HPA021141

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.    

Antibody dilution

 1:27    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020078

  

Antibody HPA021141

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human kidney shows membranous positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 42 cell types		   RNA: detected in 33 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:150  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020078

  

Antibody HPA021141

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 102.9   102.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA020078

  

Antibody HPA021141

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA020078

  

Antibody HPA021141

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 147   147  

Antigen sequence

 
SILSVKESCIANSGSDCTSHLSKDLPGIPLQNESRDSKILKGDQLLQEDY
KVNTSVPYSVSNTVVKTCNVRPPNTALDHSRKVDMQTTRKILMKKSVCLD
RHSSDEQSAPVFGKAKYTTQRMKHSSQKHNSSHFKESGHNKLSSPKI
  
LAIQQIKAVSKSLISESSQPNTSSHNISVIADLHLSTIDWEGTSFSNSPA
IQRNTFSHDLKSEVESELSAIPDGFENIPEQLSCESERYTANIKKVLDED
SDGISPEEHLLSGITDLCLQDLPLKERIFIKLSYPQDNLQPDVNLKT
 

Matching transcripts

 GEN1-001 - ENSP00000318977 [100%]
GEN1-201 - ENSP00000370653 [100%]
  GEN1-001 - ENSP00000318977 [99%]
GEN1-201 - ENSP00000370653 [99%]
 

Other gene match

    
ANTIGEN VIEW
GEN1-001
GEN1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.