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ANTIBODY INFORMATION
 
 

Antibody HPA027730

  

Antibody CAB009495

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA027730   sc-20790  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 6   4  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA027730, RRID:AB_10602494   Santa Cruz Biotechnology Cat#sc-20790, RRID:AB_2098712  

VALIDATION SUMMARY
 
 

Antibody HPA027730

  

Antibody CAB009495

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027730

  

Antibody CAB009495

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 80 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:350   1:40  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027730

  

Antibody CAB009495

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 109.7   109.7, 6.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA027730

  

Antibody CAB009495

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA027730

  

Antibody CAB009495

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 136    

Antigen sequence

 
VTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSAST
KMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVE
EKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVA
  

											 
																				

																		

										
											
Matching transcripts

												
																					ERN1-001 - ENSP00000401445 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

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RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA027730

												
										 
																				
											
											
											
Antibody CAB009495

												
										 
																		

																	

										
											
1.

												
																						Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway

	Arabi A et al

	Nat Commun 2012;3:976

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.