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Immunohistochemical staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong cytoplasmic and nuclear positivity in epidermal cells.
Expression
RNA: detected in 5 tissues Protein: detected in 7 cell types
RNA: detected in 5 tissues Protein: detected in 37 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:4500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001549
Antibody CAB025100
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420948)
Target mass (kDa)
15.1
15.1
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA001549
Antibody CAB025100
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
