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AURKB

ANTIBODY INFORMATION
 
 

Antibody HPA037708

  

Antibody CAB005862

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA037708   ab3609  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   3  

References

    

Proper citation

 Atlas Antibodies Cat#HPA037708, RRID:AB_2675623   Abcam Cat#ab3609, RRID:AB_449204  

VALIDATION SUMMARY
 
 

Antibody HPA037708

  

Antibody CAB005862

  
Immunocytochemistry

Supported    
Immunohistochemistry

 

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037708

  

Antibody CAB005862

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & midbody.    

Antibody dilution

 1:52    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037708

  

Antibody CAB005862

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of human tonsil shows strong nuclear positivity in subset of non-germinal center cells.  

Expression

   RNA: detected in 25 tissues
Protein: detected in 43 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:35  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037708

  

Antibody CAB005862

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39.5, 39.3, 35.3, 26.2   39.5, 39.3, 35.3, 34.8, 28, 26.8, 26.2, 20, 7.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA037708

  

Antibody CAB005862

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA037708

  

Antibody CAB005862

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 66    

Antigen sequence

 
WPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVME
NSSGTPDILTRHFTID
  

											 
																				

																		

										
											
Matching transcripts

												
																					AURKB-001 - ENSP00000463999 [100%] 
AURKB-005 - ENSP00000462207 [100%] 
AURKB-008 - ENSP00000462930 [100%] 
AURKB-002 - ENSP00000313950 [99%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AURKB-001
AURKB-002
AURKB-005
AURKB-008
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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