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Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, cytosol & midbody ring.
Antibody dilution
1:9
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA052555
Antibody HPA059653
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in distal tubules.
Expression
RNA: detected in 36 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
Antibody dilution
1:20
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052555
Antibody HPA059653
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
16.2, 15.5, 15.2
16.2, 15.5, 15.2, 11.5, 8.3
Antibody dilution
1:150
1:80
PROTEIN ARRAY
Antibody HPA052555
Antibody HPA059653
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
