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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.
Antibody dilution
1:29
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA040152
Antibody HPA044081
Standard validation
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells, subset of glial cells and neuropil displays strong cytoplasmic staining while endothelial cells are moderately stained.
Expression
RNA: detected in 6 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
Antibody dilution
1:300
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040152
Antibody HPA044081
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
63.7, 36.3
63.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA040152
Antibody HPA044081
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.