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APOBEC3B

ANTIBODY INFORMATION
 
 

Antibody HPA056980

  

Antibody HPA066719

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA056980   HPA066719  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 APOBEC3A - ENSG00000128383 [100%]
   APOBEC3A - ENSG00000128383 [100%]
  

Released in version

 16   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA056980, RRID:AB_2683298   Atlas Antibodies Cat#HPA066719, RRID:AB_2685711  

VALIDATION SUMMARY
 
 

Antibody HPA056980

  

Antibody HPA066719

  
Immunocytochemistry

Supported   Approved  
Western blot

Uncertain   Supported  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA056980

  

Antibody HPA066719

  

Standard validation

 Supported   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:4   1:109  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA056980

  

Antibody HPA066719

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 57.2, 45.9, 43.1, 23   57.2, 45.9, 23  

Antibody dilution

 1:80   1:360  

PROTEIN ARRAY
 
 

Antibody HPA056980

  

Antibody HPA066719

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:4350  

ANTIGEN INFORMATION
 
 

Antibody HPA056980

  

Antibody HPA066719

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 39   25  

Antigen sequence

 
ISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDP
  
ELRFLDLVPSLQLDPAQIYRVTWFI
 

Matching transcripts

 APOBEC3B-001 - ENSP00000327459 [100%]
APOBEC3B-002 - ENSP00000385060 [100%]
APOBEC3B-003 - ENSP00000385068 [100%]
  APOBEC3B-001 - ENSP00000327459 [100%]
APOBEC3B-002 - ENSP00000385060 [100%]
 

Other gene match

 APOBEC3A - ENSG00000128383 [100%]
   APOBEC3A - ENSG00000128383 [100%]
  
ANTIGEN VIEW
APOBEC3B-001
APOBEC3B-002
APOBEC3B-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.