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ANTIBODY INFORMATION
 
 

Antibody HPA034996

  

Antibody HPA034997

  

Antibody CAB004648

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA034996   HPA034997   H00008575-M01  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   13   3  

References

      

Proper citation

 Atlas Antibodies Cat#HPA034996, RRID:AB_2674402   Atlas Antibodies Cat#HPA034997, RRID:AB_2674403   Abnova Corporation Cat#H00008575-M01, RRID:AB_425767  

VALIDATION SUMMARY
 
 

Antibody HPA034996

  

Antibody HPA034997

  

Antibody CAB004648

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry


Validated
  

Validated
   Approved  
Western blot

Uncertain  

Validated
   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA034996

  

Antibody HPA034997

  

Antibody CAB004648

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.    

Antibody dilution

 1:169   1:125    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA034996

  

Antibody HPA034997

  

Antibody CAB004648

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA034997 across 73 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA034996 across 73 cell types.		    
 

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 23 cell types		   RNA: detected in 37 tissues
Protein: detected in 37 cell types		   RNA: detected in 37 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:1400   1:6000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA034996

  

Antibody HPA034997

  

Antibody CAB004648

  

Formal validation: Genetic

  
Validated

Downregulation visible in one of two siRNA lanes		    

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

   34.4, 33.1, 31.6    

Loading control

   Total protein image    

Antibody dilution

   1:279    
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 34.4   34.4, 33.1, 31.6   34.4, 33.1, 31.6, 12  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA034996

  

Antibody HPA034997

  

Antibody CAB004648

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA034996

  

Antibody HPA034997

  

Antibody CAB004648

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 70   89    

Antigen sequence

 
MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIP
VYECERSDVQIHVPTFTFRV
  
KLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQEL
AIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGK
  

											 
																				

																		

										
											
Matching transcripts

												
																					PRKRA-001 - ENSP00000318176 [100%] 

											 
																						PRKRA-001 - ENSP00000318176 [100%] 
PRKRA-003 - ENSP00000430604 [100%] 
PRKRA-009 - ENSP00000393883 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PRKRA-001
PRKRA-003
PRKRA-009
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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