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Antibody staining overlaps with antibody HPA053012.
Validated
Antibody staining overlaps with antibody HPA021563.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:49
1:26
1:200
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA021563
Antibody HPA053012
Antibody CAB012226
Antibody CAB016388
Antibody CAB022311
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 80 cell types
RNA: detected in 37 tissues Protein: detected in 75 cell types
RNA: detected in 37 tissues Protein: detected in 54 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:20
1:900
1:1750
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021563
Antibody HPA053012
Antibody CAB012226
Antibody CAB016388
Antibody CAB022311
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
218.2, 217.2
218.2, 217.2
218.2, 217.2, 63.6
218.2, 217.2, 63.6
218.2, 217.2, 63.6
Antibody dilution
1:250
1:130
1:500
1:500
1:500
PROTEIN ARRAY
Antibody HPA021563
Antibody HPA053012
Antibody CAB012226
Antibody CAB016388
Antibody CAB022311
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
