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POLR2A
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    TISSUE

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    CELL

  • PATHOLOGY

ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA021563

 

Antibody HPA053012

 

Antibody CAB012226

 

Antibody CAB016388

 

Antibody CAB022311

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Abcam plc
  Santa Cruz Biotechnology
  Chemicon
 

Product name

HPA021563   HPA053012   ab5095   sc-47701   CBL221  

Host species

Rabbit   Rabbit   Rabbit   Mouse   Mouse  

Clonality

pAb   pAb   msAb   mAb   mAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Protein A/G   Protein A/G  

Other gene match

         

Released in version

5   12   4   4   5  

References

         

Proper citation

Atlas Antibodies Cat#HPA021563, RRID:AB_1855526   Atlas Antibodies Cat#HPA053012, RRID:AB_2682017   Abcam Cat#ab5095, RRID:AB_304749   Santa Cruz Biotechnology Cat#sc-47701, RRID:AB_677353    

VALIDATION SUMMARY

 
 

Antibody HPA021563

 

Antibody HPA053012

 

Antibody CAB012226

 

Antibody CAB016388

 

Antibody CAB022311

 
Immunocytochemistry


Validated
 

Validated
  Supported   Supported    
Immunohistochemistry

Supported     Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Supported   Supported   Uncertain  
Protein array

Supported   Supported        

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA021563

 

Antibody HPA053012

 

Antibody CAB012226

 

Antibody CAB016388

 

Antibody CAB022311

 

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA053012.
 
Validated

Antibody staining overlaps with antibody HPA021563.
       
 

Standard validation

Supported   Supported   Supported   Supported    

         

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.    

Antibody dilution

1:49   1:26   1:200   1:200    

Literature conformity

The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA021563

 

Antibody HPA053012

 

Antibody CAB012226

 

Antibody CAB016388

 

Antibody CAB022311

 

Standard validation

Supported     Supported   Supported   Supported  

         

Figure description

Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.     Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.  

Expression

RNA: detected in 37 tissues
Protein: detected in 58 cell types
    RNA: detected in 37 tissues
Protein: detected in 80 cell types
  RNA: detected in 37 tissues
Protein: detected in 75 cell types
  RNA: detected in 37 tissues
Protein: detected in 54 cell types
 

Retrieval

HIER pH6     HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

1:20     1:900   1:1750   1:100  

Literature conformity

Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA021563

 

Antibody HPA053012

 

Antibody CAB012226

 

Antibody CAB016388

 

Antibody CAB022311

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
  Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
  Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
  Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
  Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.
 

         

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
   

Target mass (kDa)

218.2, 217.2   218.2, 217.2   218.2, 217.2, 63.6   218.2, 217.2, 63.6   218.2, 217.2, 63.6  

Antibody dilution

1:250   1:130   1:500   1:500   1:500  

PROTEIN ARRAY

 
 

Antibody HPA021563

 

Antibody HPA053012

 

Antibody CAB012226

 

Antibody CAB016388

 

Antibody CAB022311

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
  Supported

Pass with single peak corresponding to interaction only with its own antigen.
       

         

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

1:3000   1:1050        

ANTIGEN INFORMATION

 
 

Antibody HPA021563

 

Antibody HPA053012

 

Antibody CAB012226

 

Antibody CAB016388

 

Antibody CAB022311

 

Antigen

Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide   Not known   Native protein  

Length (aa)

129   111        

Antigen sequence

IMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGH
TIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQ
TFENQVNRILNDARDKTGSSAQKSLSEYN
 
GFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRC
IESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILE
TDGVSLMRVLS
 
 
 
 

Matching transcripts

POLR2A-001 - ENSP00000480158 [100%]
POLR2A-201 - ENSP00000483957 [100%]
  POLR2A-001 - ENSP00000480158 [100%]
POLR2A-201 - ENSP00000483957 [100%]
       

Other gene match

         
ANTIGEN VIEW
POLR2A-001
POLR2A-201
 

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