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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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POLR2A
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    TISSUE

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    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

POLR2A

Gene description

Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

Protein class

Enzymes
Predicted intracellular proteins
RNA polymerase related proteins

Predicted localization

Intracellular

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Ubiquitous nuclear expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Supported based on 4 antibodies.
HPA021563 , CAB012226 , CAB016388 , CAB022311
SHOW MORE

PROGNOSTIC SUMMARY

Gene product is not prognostic.
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA021563
CAB012226
CAB016388
CAB022311

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues displayed weak to moderate positivity.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer cells showed moderate to strong nuclear immunoreactivity.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Strong nuclear and occasional cytoplasmic positivity was seen in cancer cells.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Carcinoids and testicular cancers showed moderate to strong nuclaer positivity. Several gliomas, prostate, cervical, endometrial, skin and urotheila cancers displayed moderate nuclear staining. Remaining cancer tissus were in general weakly stained or negative.

GENE INFORMATION

Gene name

POLR2A (HGNC Symbol)

Synonyms

POLR2, POLRA, RPB1

Description

Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa (HGNC Symbol)

Entrez gene summary

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]

Chromosome

17

Cytoband

p13.1

Chromosome location (bp)

7484366 - 7514618

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000181222 (version 83.38)

Entrez gene

5430

UniProt

P24928 (UniProt - Evidence at protein level)

neXtProt

NX_P24928

Antibodypedia

POLR2A antibodies

PROTEIN BROWSER

POLR2A-001
POLR2A-002
POLR2A-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

POLR2A-001
ENSP00000480158
ENST00000617998
A0A087WWE2 [Direct mapping]
DNA-directed RNA polymerase
Show all
Predicted intracellular proteins
RNA polymerase related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001047 [core promoter binding]
GO:0001055 [RNA polymerase II activity]
GO:0003677 [DNA binding]
GO:0003899 [DNA-directed RNA polymerase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005665 [DNA-directed RNA polymerase II, core complex]
GO:0005719 [nuclear euchromatin]
GO:0006351 [transcription, DNA-templated]
GO:0006366 [transcription from RNA polymerase II promoter]
GO:0014070 [response to organic cyclic compound]
GO:0071453 [cellular response to oxygen levels]
Show all
1980 aa
218.2 kDa
No 0
POLR2A-002
ENSP00000461879
ENST00000572844
P24928 [Direct mapping]
DNA-directed RNA polymerase II subunit RPB1
Show all
Enzymes
   ENZYME proteins
   Transferases
Predicted intracellular proteins
RNA polymerase related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000398 [mRNA splicing, via spliceosome]
GO:0000974 [Prp19 complex]
GO:0001055 [RNA polymerase II activity]
GO:0001172 [transcription, RNA-templated]
GO:0003677 [DNA binding]
GO:0003899 [DNA-directed RNA polymerase activity]
GO:0003968 [RNA-directed RNA polymerase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005665 [DNA-directed RNA polymerase II, core complex]
GO:0006281 [DNA repair]
GO:0006283 [transcription-coupled nucleotide-excision repair]
GO:0006289 [nucleotide-excision repair]
GO:0006351 [transcription, DNA-templated]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0006366 [transcription from RNA polymerase II promoter]
GO:0006367 [transcription initiation from RNA polymerase II promoter]
GO:0006368 [transcription elongation from RNA polymerase II promoter]
GO:0006370 [7-methylguanosine mRNA capping]
GO:0008380 [RNA splicing]
GO:0010467 [gene expression]
GO:0016032 [viral process]
GO:0031047 [gene silencing by RNA]
GO:0031625 [ubiquitin protein ligase binding]
GO:0033120 [positive regulation of RNA splicing]
GO:0034587 [piRNA metabolic process]
GO:0035019 [somatic stem cell population maintenance]
GO:0044822 [poly(A) RNA binding]
GO:0046872 [metal ion binding]
GO:0050434 [positive regulation of viral transcription]
Show all
566 aa
63.6 kDa
No 0
POLR2A-201
ENSP00000483957
ENST00000621442
A0A0C4DGZ0 [Direct mapping]
DNA-directed RNA polymerase
Show all
Predicted intracellular proteins
RNA polymerase related proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001055 [RNA polymerase II activity]
GO:0003677 [DNA binding]
GO:0003899 [DNA-directed RNA polymerase activity]
GO:0005665 [DNA-directed RNA polymerase II, core complex]
GO:0006351 [transcription, DNA-templated]
GO:0006366 [transcription from RNA polymerase II promoter]
Show all
1970 aa
217.2 kDa
No 0

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