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Immunohistochemical staining of human rectum shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 34 tissues Protein: detected in 51 cell types
RNA: detected in 34 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014703
Antibody HPA073662
Antibody CAB013063
Standard validation
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420015)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
22.4, 20.7, 15.2, 15.1, 13.6
22.4
22.4, 20.7, 15.2, 15.1, 13.6, 10.5
Antibody dilution
1:125
1:230
1:500
PROTEIN ARRAY
Antibody HPA014703
Antibody HPA073662
Antibody CAB013063
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
