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Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
Antibody dilution
1:72
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA043752
Antibody HPA050345
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA050345 across 71 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA043752 across 71 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 67 cell types
RNA: detected in 37 tissues Protein: detected in 60 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:60
1:70
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043752
Antibody HPA050345
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406128)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
20.6, 16.9
20.6
Antibody dilution
1:125
1:360
PROTEIN ARRAY
Antibody HPA043752
Antibody HPA050345
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
