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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Antibody dilution
1:92
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA003303
Antibody HPA028872
Antibody HPA030445
Antibody CAB073417
Standard validation
Approved
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
Immunohistochemical staining of human nasopharynx shows moderate nuclear positivity in respiratory epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 42 cell types
RNA: detected in 37 tissues Protein: detected in 77 cell types
RNA: detected in 37 tissues Protein: detected in 44 cell types
RNA: detected in 37 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:75
1:300
1:15
1:6000
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003303
Antibody HPA028872
Antibody HPA030445
Antibody CAB073417
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405443)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405443)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
