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LIMK2

ANTIBODY INFORMATION
 
 

Antibody HPA008183

  

Antibody HPA053882

  

Antibody CAB019313

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA008183   HPA053882   2867.00.02  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

   PPP1R14B - ENSG00000173457 [100%]
    

Released in version

 3   12   5  

References

 2      

Proper citation

 Atlas Antibodies Cat#HPA008183, RRID:AB_1079252   Atlas Antibodies Cat#HPA053882, RRID:AB_2682291    

VALIDATION SUMMARY
 
 

Antibody HPA008183

  

Antibody HPA053882

  

Antibody CAB019313

  
Immunocytochemistry

    Supported  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008183

  

Antibody HPA053882

  

Antibody CAB019313

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.  

Antibody dilution

     1:13  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008183

  

Antibody HPA053882

  

Antibody CAB019313

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:100   1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008183

  

Antibody HPA053882

  

Antibody CAB019313

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 77.9, 72.2, 71.2, 69.9   77.9, 71.2, 15.9, 8.1   77.9, 72.2, 71.2, 69.9, 9.4  

Antibody dilution

 1:250   1:450   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008183

  

Antibody HPA053882

  

Antibody CAB019313

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3600    

ANTIGEN INFORMATION
 
 

Antibody HPA008183

  

Antibody HPA053882

  

Antibody CAB019313

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 107   27    

Antigen sequence

 
VEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHAL
STLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLR
CSSSYSQ
  
ELEIDVDELLDMESDDARAARVKELLV
  

											 
																				

																		

										
											
Matching transcripts

												
																					LIMK2-001 - ENSP00000332687 [100%] 
LIMK2-002 - ENSP00000384602 [100%] 
LIMK2-003 - ENSP00000339916 [100%] 
LIMK2-004 - ENSP00000330470 [100%] 

											 
																						LIMK2-002 - ENSP00000384602 [93%] 
LIMK2-003 - ENSP00000339916 [93%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						PPP1R14B - ENSG00000173457 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LIMK2-001
LIMK2-002
LIMK2-003
LIMK2-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008183

												
										 
																				
											
											
											
Antibody HPA053882

												
										 
																				
											
											
											
Antibody CAB019313

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission

	Kim JE et al

	Cell Death Differ 2014;21(7):1036-49

			Application: ICC-IF, WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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