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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subsets of cells.
Expression
RNA: detected in 33 tissues Protein: detected in 12 cell types
RNA: detected in 33 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:350
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003494
Antibody CAB003816
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
61.2, 58, 36, 31.6, 15.3
61.2, 58.3, 58, 36, 31.6, 22.2, 17.8, 16.2, 15.3
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003494
Antibody CAB003816
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
